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CP53 Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

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Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
CP53-100UG
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      Ampoule plast. 100 μg
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      Description
      OverviewRecognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.
      Catalogue NumberCP53
      Brand Family Calbiochem®
      SynonymsAnti-Inter-Cellular Adhesion Molecule, Anti-CD54
      References
      ReferencesTakami, S., et al. 1998. Circ. 97, 721.
      Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
      DeGraba, T.J. 1997. Neurology 49, S15.
      Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
      McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
      Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
      De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
      Fawcett, J., et al. 1992. Nature 360, 481.
      Vazeux, R. et al. 1992. Nature 360, 485.
      Boyd, A.W., et al. 1989. Blood 73, 1896.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlEndothelial cells or tonsil tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.
      Key Applications Cell Inhibition Assays
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Paraffin Sections
      Application NotesCell Inhibition Assays (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (1:50-1:100)
      Immunoblotting (1:200-1:400)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenRaji cells
      ImmunogenHuman
      CloneW-CAM-1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Global Trade Item Number
      Référence GTIN
      CP53-100UG 04055977221008