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MABN1578 Anti-GNAT1 Antibody, clone 11A8.1

Anti-GNAT1 Antibody, clone 11A8.1 is an antibody against GNAT1 for use in Western Blotting.

Anti-GNAT1 Antibody, clone 11A8.1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: G-proteins Gene Symbol: GNAT1, GNATR, CSNB1G, CSNBAD3 UniProt Number: P11488
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MABN1578
100 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1578
      DescriptionAnti-GNAT1 Antibody, clone 11A8.1
      Alternate Names
      • Guanine nucleotide-binding protein G(t) subunit alpha-1
      • Transducin alpha-1 chain
      Background InformationGuanine nucleotide-binding protein G(t) subunit alpha-1 (UniProt P11488; also known as Transducin alpha-1 chain) is encoded by the GNAT1 (also known as GNATR, CSNB1G, CSNBAD3) gene (Gene ID 2779) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (α), beta (β), and gamma (γ) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyze the GDP-to-GTP exchange of the Gα subunit, causing a dissociation of the Gβγ dimer from the now GTP-bound Gα subunit. The Gα subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gβγ and re-starts the cycle. There exist at least 20 different Gα subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Eight different genes code for Gi alpha subunits, including GNAT1 and GNAT2 that code for Gt in the rod (rTα by GNAT1) and cone (cTα by GNAT2) cells of the retina, where they function as the alpha subunit of the trimeric G protein transducin. The α-subunit binds GTP and activates cyclic GMP phosphodiesterase (PDE), whereas the β and γ subunits mediate interaction with rhodopsin. Mutations in RHO, PDE6B, and GNAT1 genes are associated with autosomal dominant congenital stationary night blindness (CSNB), and patients with mutations in these genes exhibit the Riggs- or Schubert-Bornschein-type electronegative electroretinogram (ERG).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GNAT1 Antibody, clone 11A8.1 is an antibody against GNAT1 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human GNAT1 internal fragment.
      EpitopeInternal.
      Clone11A8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 11A8.1 was raised against human Gt alpha-1 subunit (GNAT1) recombinant fragment. Cross-reactivity toward human Gt alpha-2 subunit (GNAT2) has not been determined.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GNAT1
      • GNATR
      • CSNB1G
      • CSNBAD3
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~40 kDa observed. 39.91 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human retina tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected GNAT1 in 10 µg of human retina tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABN1578 04055977351101