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CB1031 Anti-GHRL Mouse mAb (2F4)

Anti-GHRL Mouse mAb (2F4) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Ghrelin

CB1031
  
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      This product has been discontinued.





      Recognizes the ~12 kDa GHRL protein in HeLa cells and human stomach tissue.
      Catalogue NumberCB1031
      Brand Family Calbiochem®
      SynonymsAnti-Ghrelin
      Application Data
      Detection of human GHRL by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GHRL by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, Human stomach tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml))
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human rGHRL (aa 1-118), expressed as a GST fusion protein
      ImmunogenNA
      Clone2F4
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      CB1031 0

      Documentation

      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Ghrelin
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml))
      Application Data
      Detection of human GHRL by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GHRL by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-GHRL Mouse mAb (2F4) (Cat. No. CB1031) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antiboy. Recognizes the ~12 kDa GHRL protein.
      BackgroundThis gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.
      HostMouse
      Immunogen speciesNA
      ImmunogenFull-length, recombinant human rGHRL (aa 1-118), expressed as a GST fusion protein
      Clone2F4
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells, Human stomach tissue
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review