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09-860 Anti-DDX3X Antibody

Use Anti-DDX3X Antibody (Rabbit Polyclonal Antibody) validated in WB to detect DDX3X also known as ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal.

Anti-DDX3X Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Binding Protein (RBP) Gene Symbol: CAP-Rf , DBX , DDX14 , DDX3, EC 3.6.1.- , HLP2 UniProt Number: O00571
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09-860
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number09-860
      DescriptionAnti-DDX3X Antibody
      Alternate Names
      • ATP-dependent RNA helicase DDX3X
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
      • DEAD box protein 3, X-chromosomal
      • DEAD box, X isoform
      • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
      • DEAD/H box-3
      • Helicase-like protein 2
      • helicase like protein 2
      Background InformationDDX3X, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked, is a member of the DEAD box family of proteins. Characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), DEAD box proteins are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3X is an ATP-dependent RNA helicase and acts as a co-factor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. DDX3X is also involved in HIV-1 replication and interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
      References
      Product Information
      FormatSerum
      Control
      • A431 cell lysate
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-DDX3X Antibody (Rabbit Polyclonal Antibody) validated in WB to detect DDX3X also known as ATP-dependent RNA helicase DDX3X, DEAD box protein 3 X-chromosomal.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic linear peptide corresponding to DDX3X.
      HostRabbit
      SpecificityThis antibody detects RNA-binding protein DDX3X.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq].
      Gene Symbol
      • CAP-Rf
      • DBX
      • DDX14
      • DDX3
      • EC 3.6.1.-
      • HLP2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

      SUBUNIT STRUCTURE: Found in a complex with Rev and XPO1. Interacts with XPO1 and TDRD3. Interacts with HCV core protein.

      SUBCELLULAR LOCATION: Nucleus speckle. Cytoplasm. Note: Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner.

      SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.

      Contains 1 helicase ATP-binding domain.

      Contains 1 helicase C-terminal domain.
      Molecular Weight~73 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on A431 cell lysate.

      Western Blot Analysis: 1:1000 dilution of this antibody detected DDX3X in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      09-860 04053252744440

      Documentation

      Anti-DDX3X Antibody FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-DDX3X Antibody Certificats d'analyse

      TitreNuméro de lot
      Anti-DDX3X - 2430960 2430960
      Anti-DDX3X - 2452490 2452490
      Anti-DDX3X - 2325057 2325057
      Anti-DDX3X - 3200651 3200651
      Anti-DDX3X - 3321057 3321057
      Anti-DDX3X - 3862683 3862683
      Anti-DDX3X - NG1619915 NG1619915
      Anti-DDX3X - NG1895575 NG1895575
      Anti-DDX3X -2492823 2492823
      Anti-DDX3X -2714517 2714517
      Show more

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells.
      Vella, P; Barozzi, I; Cuomo, A; Bonaldi, T; Pasini, D
      Nucleic acids research  40  3403-18  2011

      Afficher le résumé       		22210892 22210892