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MABF2108-100UG Anti-Chlamydial HSP60 Antibody, clone A57-B9

Anti-Chlamydial HSP60, clone A57-B9, Cat. No. MABF2108, is a mouse monoclonal antibody that detects Chlamydial HSP60 and has been tested for use in ELISA, Immunofluorescence, Immunocytochemistry, and Western Blotting.

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MABF2108-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2108-100UG
      DescriptionAnti-Chlamydial HSP60 Antibody, clone A57-B9
      Alternate Names
      • Chlamydia heat shock protein 60
      Background InformationChlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Clone A57-B9 detects Chlamydial HSP60 (GroEL). It reacts with the short peptide of 6 amino acids from the carboxyl terminal region of HSP60. HSP60 facilitates the correct folding of imported proteins and may prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Chlamydial HSP60, clone A57-B9, Cat. No. MABF2108, is a mouse monoclonal antibody that detects Chlamydial HSP60 and has been tested for use in ELISA, Immunofluorescence, Immunocytochemistry, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).

      Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).

      ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).

      Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
      Biological Information
      ImmunogenRecombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
      CloneA57-B9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      Species Reactivity NoteBacteria (Chlamydia genus)
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HeLa cells infected with chlamydia trachomatis serovar L2 (LGV 434 L2) .

      Western Blotting Analysis: 2 µg/mL of this antibody detected Chlamydial HSP60 in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2) .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2108-100UG 04054839636455