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217669 Anti-CD28 Mouse mAb (ANC28.1/5D10)

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Anti-CD28 Mouse mAb (ANC28.1/5D10) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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217669
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Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Prix & Disponibilité

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217669-100UG
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      Description
      OverviewRecognizes CD28 on human T cells and BHK cells transfected with human CD28.
      Catalogue Number217669
      Brand Family Calbiochem®
      References
      ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
      61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
      Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Preservative500 µg/ml gentamycin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      T Cell Co-stimulation
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      T cell Co-stimulation (see comments)
      Application CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
      ImmunogenHuman
      CloneANC28.1/5D10
      HostMouse
      Purity>95% immunoglobulin by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Global Trade Item Number
      Référence GTIN
      217669-100UG 04055977202953