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AB3121 Anti-Angiopoietin-2 Antibody, NT

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AB3121
50 µg  
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      Replacement Information

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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3121
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Angiopoietin-2 Antibody, NT
      Alternate Names
      • Ang-2
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5
      Quality LevelMQ100
      Applications
      ApplicationDetect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

      ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

      The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
      Gene Symbol
      • ANGPT2
      • ANG-2
      • Tie2-ligand
      • ANG2
      • angiopoietin-2a
      • AGPT2
      • Ang2
      • angiopoietin-2B
      • angiopoietin-2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15123 # Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
      SIZE: 496 amino acids; 56919 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: O15123 ## Contains 1 fibrinogen C-terminal domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AB3121 04053252364679

      Documentation

      Anti-Angiopoietin-2 Antibody, NT FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Angiopoietin-2 Antibody, NT Certificats d'analyse

      TitreNuméro de lot
      RABBIT ANTI-MOUSE ANGIOPOIETIN-2 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2462718 2462718
      RABBIT ANTI-MOUSE ANGIOPOIETIN-2 - 3675865 3675865
      RABBIT ANTI-MOUSE ANGIOPOIETIN-2 -2558404 2558404
      RABBIT ANTI-MOUSE ANGIOPOIETIN-2 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2201356 2201356
      RABBIT ANTI-MOUSE ANGIOPOIETIN-2 AFFINITY-PURIFIED POLYCLONAL ANTIBODY - 2274027 2274027

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Induction of angiopoietin-2 after spinal cord injury.
      Durham-Lee, JC; Wu, Y; Mokkapati, VU; Paulucci-Holthauzen, AA; Nesic, O
      Neuroscience  202  454-64  2011

      Afficher le résumé
      22020092 22020092
      Sertoli Cell-Initiated Testicular Innate Immune Response through Toll-Like Receptor-3 Activation Is Negatively Regulated by Tyro3, Axl, and Mer Receptors.
      Sun B, Qi N, Shang T, Wu H, Deng T, Han D
      Endocrinology  2009

      Afficher le résumé
      20363878 20363878
      Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
      Makinde TO, Agrawal DK
      Am J Respir Cell Mol Biol  2009

      Afficher le résumé
      20463289 20463289
      Conditional activation of FGFR1 in the prostate epithelium induces angiogenesis with concomitant differential regulation of Ang-1 and Ang-2.
      Winter, SF; Acevedo, VD; Gangula, RD; Freeman, KW; Spencer, DM; Greenberg, NM
      Oncogene  26  4897-907  2007

      Afficher le résumé
      17297442 17297442
      Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning.
      Davis, S, et al.
      Cell, 87: 1161-9 (1996)  1996

      8980223 8980223
      Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis.
      Suri, C, et al.
      Cell, 87: 1171-80 (1996)  1996

      8980224 8980224