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MABF2147-100UG Anti-Aminopeptidase N (CD13) Antibody, clone 452

Anti-Aminopeptidase N (CD13), clone 452, Cat. No. MABF2147, is a mouse monoclonal antibody that detects Aminopeptidase N and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist function, and Western Blotting.

MABF2147-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2147-100UG
      DescriptionAnti-Aminopeptidase N (CD13) Antibody, clone 452
      Alternate Names
      • AP-N
      • hAPN
      • Alanyl aminopeptidase
      • Aminopeptidase M
      • AP-M
      • Microsomal aminopeptidase
      • Myeloid plasma membrane glycoprotein CD13
      • gp150
      • CD13
      Background InformationAminopeptidase N (UniProt: P15144; also known as EC:3.4.11.2, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, CD13) is encoded by the ANPEP (also known as APN, CD13, PEPN) gene (Gene ID: 290) in human. Aminopeptidase N is a single-pass type II membrane protein that is also found as a soluble form in cells. It is widely expressed as a homodimer of 280 kDa on the cell surface in many tissues, including intestinal epithelia and the nervous system. It serves as a broad specificity aminopeptidase that plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. It preferentially cleaves N-terminus neutral amino acids, most notably alanine residue. Aminopeptidase N is involved in many physiological processes, including antigen presentation regulation, differentiation, proliferation, apoptosis, cancer metastasis, and angiogenesis. It is also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Human Aminopeptidase N consists of a short N-terminal cytoplasmic end (aa 2-8), a transmembrane domain (aa 9-32), and a large extracellular portion (aa 33-967), which is composed of a Ser/Thr-rich region (aa 33-68) and the metalloprotease domain (aa 69-967).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-Aminopeptidase N (CD13), clone 452, Cat. No. MABF2147, is a mouse monoclonal antibody that detects Aminopeptidase N and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Agonist function, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Agonist or Inhibitor
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected Aminopeptidase N (CD13) in Immunocytochemistry applications (Rahman, M.M., et. al. (2014). Front Physiol. 4:402; Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).

      Flow Cytometry Analysis: A representative lot detected Aminopeptidase N (CD13) in Flow Cytometry applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).

      Western Blotting Analysis: A representative lot detected Aminopeptidase N (CD13) in Western Blotting applications (Rahman, M.M., et. al. (2014). Stem Cells. 32(6):1564-77; Rahman, M.M., et. al. (2014). Front Physiol. 4:402).

      Agonist Analysis: A representative lot induced a temporal increase in phosphorylation of FAK, ERK and Src kinases in U937 cells. (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Aminopeptidase N (CD13) in Immunoprecipitation applications (Subramani, J., et. al. (2013). J Immunol. 191(7):3905-12).
      Biological Information
      ImmunogenIsolated human dermal fibroblasts.
      Clone452
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 452 specifically detects Aminopeptidase N (CD13) in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ANPEP
      • APN
      • CD13
      • PEPN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight109.54 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in U937 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected Aminopeptidase N (CD13) in one million U937 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2147-100UG 04054839577055

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