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AP1137 Anti-ADA Mouse mAb (4G4-1C6)

AP1137
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ADA protein.
      Catalogue NumberAP1137
      Brand Family Calbiochem®
      SynonymsAnti-Adenosine aminohydrolase, Adenosine deaminase
      Application Data
      Detection of human ADA by immunoblotting. Samples: Whole cell lysate from HL-60 cells. Primary antibody: Anti-ADA Mouse mAb (4G4-1C6) (Cat. No. AP1137) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADA by immunoblotting. Samples: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADA Mouse mAb (4G4-1C6) (Cat. No. AP1137) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull length recombinant human ADA (aa1-364) expressed as a GST fusion protein
      Clone4G4-1C6
      HostMouse
      IsotypeIgG1
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AP1137 0

      Documentation

      Anti-ADA Mouse mAb (4G4-1C6) Certificats d'analyse

      TitreNuméro de lot
      AP1137
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-October-2010 JLN
      SynonymsAnti-Adenosine aminohydrolase, Adenosine deaminase
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ADA by immunoblotting. Samples: Whole cell lysate from HL-60 cells. Primary antibody: Anti-ADA Mouse mAb (4G4-1C6) (Cat. No. AP1137) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADA by immunoblotting. Samples: Whole cell lysate from Jurkat cells. Primary antibody: Anti-ADA Mouse mAb (4G4-1C6) (Cat. No. AP1137) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes ADA protein.
      BackgroundADA (Adenosine deaminase) is an enzyme involved in the purine catabolic pathway. It irreversibly deaminates adenosine, thereby converting it into the related nucleoside, inosine. Defects in ADA are a cause of autosomal recessive severe combined immuno-deficiency (SCID).
      HostMouse
      ImmunogenFull length recombinant human ADA (aa1-364) expressed as a GST fusion protein
      Clone4G4-1C6
      IsotypeIgG1
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review