Millipore Sigma Vibrant Logo

NA81 Anti-5-Methylcytosine Mouse mAb (162 33 D3)

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Anti-5-Methylcytosine Mouse mAb (162 33 D3) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-5-mc, Anti-5-MeCyd

View Products on Sigmaaldrich.com
NA81
Voir les Prix & la Disponibilité

Aperçu

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
NA81-50UG
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 50 μg
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Ajouter aux favoris
      Description
      OverviewRecognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

      Specific
      • Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
      • Flow Cytometry
      • Frozen Sections
      • Immunoblotting
      • Immunofluorescence
      • Paraffin Sections
      Reliable
      • Generates reproducible results.
      • Clone 162 33 D3 has been used in over 50 publications.

      Catalogue NumberNA81
      Brand Family Calbiochem®
      SynonymsAnti-5-mc, Anti-5-MeCyd
      Application Data
      Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB

      Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.
      References
      ReferencesEhrlich, M. 2002. Oncogene 21, 5400. (Review)
      Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
      Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
      Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
      Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
      Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
      De Capoa, A., et al. 1999. FASEB J. 13, 89.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      Positive controlNIH3T3 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesParaffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Radioimmunoassay
      Southwestern Blot
      Application NotesELISA (0.1 µg/ml)
      Flow Cytometry (1 µg/ml)
      Frozen Sections (5-10 µg/ml)
      Immunoblotting (5 µg/ml)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (5-10 µg/ml)
      Radioimmunoassay (0.1 µg/ml)
      Southwestern Blot (5 µg/ml)
      Application CommentsClone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
      Biological Information
      Immunogen5-methylcytosine conjugated to ovalbumin
      Clone162 33 D3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Référence GTIN
      NA81-50UG 04055977209792