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UFC910008 Amicon Ultra-15, PLHK, membrane Ultracel-PL, 100 kD

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Principales applications: Extraction de protéines, Purification de protéines
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UFC910008
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Key ApplicationsNMWLVolumeVolume minimal du concentrat final
      Extraction de protéines, Purification de protéines100 KDa15 mL200 µL
      Description
      RéférenceUFC910008
      Nom de marque
      • Amicon Ultra
      DescriptionAmicon Ultra-15, PLHK, membrane Ultracel-PL, 100 kD
      Informations généralesAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
      UFC910008 - 8 pack
      UFC910024 - 24 pack
      UFC910096 - 96 pack



      High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
      High retentate recovery of greater than 90%
      Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
      Heat-sealed membrane minimizes downstream extractables
      100% integrity tested for reliable performance
      Convenient sample monitoring with translucent housing and volume gradations
      Direct pipettor sample access eliminates processing step to recover concentrate
      High concentration factors of 80–100X

      Applications:
      Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
      Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
      Concentration of dilute or pre-purified proteins from column eluents
      Desalting, buffer exchange and protein dialysis
      Références bibliographiques
      Informations produit
      Code du filtrePLHK
      Code couleurTransparent avec bouchon rose
      Type de filtreUltrafiltration
      PMNL100 KDa
      Quality LevelMQ400
      Applications
      ApplicationUltracel-100 regenerated cellulose membrane, 15 mL sample volume
      Principales applications
      • Extraction de protéines
      • Purification de protéines
      Notes opératoiresRotor à godets mobiles : 4000 x g ; Rotor à angle fixe (35°) : 5000 x g
      Informations biologiques
      StérilitéNon stérile
      Informations physico-chimiques
      Application de centrifugationConcentration et dessalage de protéines
      Dimensions
      Longueur12.1 cm (4.807 in.)
      Diamètre2.97 cm
      Surface de filtration7.6 cm²
      Volume minimal du concentrat final200 µL
      Volume15 mL
      Informations sur les matériaux
      Chimie
      • Regenerated Cellulose (RC)
      Matériau du dispositif
      • Support : Styrène/butadiène
      • Tube : Polypropylène
      • Bouchon avec revêtement interne : Polyéthylène
      Matériau du supportCellulose régénérée
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Informations relatives au stockage et à l'expédition
      Informations sur l'emballage
      Quantité8
      Informations sur le transport
      Information complémentaire
      Caractéristiques
      Global Trade Item Number
      Référence GTIN
      UFC910008 04053252504129

      Documentation

      Brochure

      Titre
      REFINE PROTEIN PREPARATION. Tools for better protein analysis

      FAQ

      QuestionRéponse
      Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
      What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
      How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
      Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
      Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
      Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
      What is the maximum sample volume for Amicon Ultra?
      Amicon Ultra-4Amicon Ultra-15
      Swinging Bucket4ml 15ml
      Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
      3.5ml(23 degree rotor angle)
      What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
      4ml15ml
      Swinging Bucket4000 x g4000 x g
      3500 x g for concentrated/viscous solutions
      Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
      What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
      Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
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      Manuels d'utilisation

      Titre
      Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide