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113000 Acridine Orange - CAS 65-61-2 - Calbiochem

Acridine Orange - CAS 65-61-2 - Calbiochem : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: AO, 3,6-bis(Dimethylamino)acridine, HCl

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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      CAS #Empirical Formula
      65-61-2C₁₇H₂₀ClN₃
      Description
      OverviewA cell-permeable, cationic fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognosis of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry that can be used to provide information about the in situ content, molecular structure, and conformation of many nucleic acid-containing cell constituents.
      Catalogue Number113000
      Brand Family Calbiochem®
      SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
      References
      ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
      Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
      Olivier, R. 1995. Methods Enzymol. 251, 270.
      Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
      Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
      Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
      El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
      Lopez, F., et al. 1991. Cytometry 12, 42.
      Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
      Hermansen, D.K., et al. 1989. Cytometry 10, 739.
      Product Information
      CAS number65-61-2
      ATP CompetitiveN
      FormBurnt-orange solid
      Hill FormulaC₁₇H₂₀ClN₃
      Chemical formulaC₁₇H₂₀ClN₃
      Hygroscopic Hygroscopic
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetInteracts with DNA and RNA
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSAR7601000
      Safety Information
      R PhraseR: 20/21/22-40

      Harmful by inhalation, in contact with skin and if swallowed.
      Limited evidence of a carcinogenic effect.
      S PhraseS: 36

      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful & Carcinogenic / Teratogenic
      Storage +15°C to +30°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      113000 0

      Documentation

      Acridine Orange - CAS 65-61-2 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      113000

      Références bibliographiques

      Aperçu de la référence bibliographique
      Gonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
      Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
      Olivier, R. 1995. Methods Enzymol. 251, 270.
      Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
      Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
      Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
      El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
      Lopez, F., et al. 1991. Cytometry 12, 42.
      Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
      Hermansen, D.K., et al. 1989. Cytometry 10, 739.

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-April-2008 RFH
      SynonymsAO, 3,6-bis(Dimethylamino)acridine, HCl
      DescriptionA cell-permeable fluorescent dye that interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, this cationic dye is spectrally very similar to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm. Upon association with RNA, the excitation maximum shifts to 460 nm and the emission maximum shifts to 650 nm (red). Often used to measure single- and double-stranded DNA and RNA in the diagnosis, classification, and prognostication of many neoplasms. A very versatile fluorescent stain used in histochemistry and cytochemistry providing information about the in situ content, molecular structure, conformation, and environment of many nucleic acid-containing cell constituents.
      FormBurnt-orange solid
      CAS number65-61-2
      RTECSAR7601000
      Chemical formulaC₁₇H₂₀ClN₃
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMF, Ethanol, or H₂O (1 mg/ml)
      Storage Protect from light
      +15°C to +30°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 1 month at 4°C.
      Toxicity Harmful & Carcinogenic / Teratogenic
      ReferencesGonzalez, K., et al. 1995. Curr. Eye Res. 14, 269.
      Muro-Cacho, C.A., et al. 1995. J. Immunol. 154, 5555.
      Olivier, R. 1995. Methods Enzymol. 251, 270.
      Busch, G.L., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 9165.
      Darzynkiewicz, Z. 1994. Methods Cell. Biol. 41, 401.
      Delic, J., et al. 1991. Exp. Cell Res. 194, 147.
      El-Naggar, A.K., et al. 1991. Cytometry 12, 330.
      Lopez, F., et al. 1991. Cytometry 12, 42.
      Gurrieri, S., et al. 1990. Biochemistry 29, 3396.
      Hermansen, D.K., et al. 1989. Cytometry 10, 739.