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71560 pTriEx™-7 DNA

pTriEx™-7 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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71560

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Description
Overview	This product has been discontinued. The pTriEx™-7 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, pTriEx-7 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For expression in E. coli, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. The pTriEx-7 contains the coding sequence for the mouse IgM signal sequence to allow secretion of the expressed protein. pTriEx-7 also encodes an N-terminal 8 aa Strep•Tag ® II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag ® coding sequence with 10 histidines. Additionally, the pTriEx-7 vector is available as an Ek/LIC Vector Kit. The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Catalogue Number	71560
Brand Family	Novagen®

Description

Overview

This product has been discontinued.

The pTriEx™-7 vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, pTriEx-7 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. For expression in E. coli, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows IPTG based induction. The pTriEx-7 contains the coding sequence for the mouse IgM signal sequence to allow secretion of the expressed protein. pTriEx-7 also encodes an N-terminal 8 aa Strep•Tag ® II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag ® coding sequence with 10 histidines. Additionally, the pTriEx-7 vector is available as an Ek/LIC Vector Kit.

The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Catalogue Number

71560

Brand Family

Novagen®

References

Product Information

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped with Blue Ice or with Dry Ice
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
71560	0

Documentation

pTriEx™-7 DNA SDS

Title
Safety Data Sheet (SDS)

pTriEx™-7 DNA Certificates of Analysis

Title	Lot Number
71560	Enter Lot Number

Brochure

Title
Insect Cell Expression

User Protocols

Title
TB053 Academic and Non-profit Laboratory Assurance Letter
TB250 pTriEx™ System Manual

Vector Map

Title
TB436 pTriEx-7 Vector Map

Vector Sequence

Title
pTriEx-7 Vector Sequence

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71560 pTriEx™-7 DNA

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Overview

Documentation

pTriEx™-7 DNA SDS

pTriEx™-7 DNA Certificates of Analysis

Brochure

User Protocols

Vector Map

Vector Sequence

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