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70933 pTriEx™-4 Neo DNA - Novagen

pTriEx™-4 Neo DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70933
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Overview

Replacement Information

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      Description
      OverviewThe pTriEx™-4 Neo vector is designed to allow rapid characterization of target genes in E. coli, insect and vertebrate cells, and to allow rapid selection of stable transfected vertebrate cells expressing high levels of target gene. Expression in vertebrate cells is mediated by the CMV immediate early enhancer and promoter2. The drug selection marker is expressed under the control of the EMC virus Cap-Independent Translation Enhancer (CITE) sequence (or IRES), allowing rapid selection of transfected vertebrate cells using the drug G418 or neomycin sulfate. For expression in insect cells, pTriEx-4 Neo contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. Expression can be induced in hosts such as NovaBlue by infecting with λCE6, a phage that constitutively expresses T7 RNA polymerase from the λlpL and λpI promoters. Alternatively, pTriEx recombinant plasmids can be transferred into a (DE3)pLacI host that allows induction with IPTG. Native protein can be expressed by cloning into the Nco I site, or generated by cloning into the PshA I or Sma I sites and cleaving the fusion protein with enterokinase or thrombin, respectively.





      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70933
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70933-3 07790788060961

      Documentation

      pTriEx™-4 Neo DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pTriEx™-4 Neo DNA - Novagen Certificates of Analysis

      TitleLot Number
      70933

      Citations

      Title
    • Saurabh K. Gupta, et al. (2007) Human T-cell leukemia virus type1 tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated huChk2. Journal of Biological Chemistry 282, 29431-29440.
    • Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.
    • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
    • Abdelhakim Ahmed-Belkacem, et al. (2005) Flavonoid structure-activity studies identify 6-prenylchrysin and tectochrysin as potent and specific inhibitors of breast cancer resistance protein ABCG2. Cancer Research 65, 4852-4860.
      		•

      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB250 pTriEx™ System Manual

      Vector Map

      Title
      TB299 pTriEx-4 Neo Vector Map

      Vector Sequence

      Title
      pTriEx-4 Neo Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors