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71719 iFOLD® Protein Refolding System 2

71719
  
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      This product has been discontinued.





      The iFOLD® Protein Refolding System 2 is a detergent-free kit to screen for optimal refolding conditions for proteins found in inclusion bodies. The kit provides a systematic evaluation of 95 conditions representing different buffering systems, pH values, and combinations of salt, redox agent, and refolding additives. The system consists of inclusion body purification reagents and a pre-dispensed 96-well plate-based matrix of refolding buffers. Following cell lysis, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of washes with a non-detergent sulfobetaine (NDSB). The purified inclusion bodies are reduced and denatured by addition of Tris(2-carboxyethyl) phosphine (TCEP) and guanidine hydrochloride and refolded by rapid dilution into the iFOLD System 2 detergent-free 96-well buffer matrix. Each kit is sufficient for screening up to 96 refolding conditions (95 experimental and 1 control well) for a single protein.
      Catalogue Number71719
      Brand Family Novagen®
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      Ship Code Multiple Storage Conditions
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
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      Global Trade Item Number
      Catalogue Number GTIN
      71719 0