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14-352-D cRAF (RAF1)

Recombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

cRAF (RAF1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-352-D
10 μg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-352-D
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptioncRAF (RAF1)
      OverviewRecombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag
      References
      Product Information
      Presentation40 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 16 mM glutathione, 20% glycerol, 3 mM DTT
      Quality LevelMQ100
      Applications
      ApplicationRecombinant human RAF1, also known as c-RAF, amino acids 306-648, containing the mutations Y340D and Y341D, with N terminal GST tag for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
      Key Applications
      • Kinase Assay
      Biological Information
      Concentration0.71 mg/ml
      Purity≥70%
      SourceSf9 insect cells
      Specific Activity158 pmol/min/µg
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • C-RAF
      • cRaf
      • Raf-1
      • c-Raf
      • CRAF
      • RAF
      Target Sub-FamilyTKL
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Kinase assay: 10 μM ATP, 1 μM MEK1(K97R), 1 hour.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for > 6 month at –80°C from date of receipt.
      Packaging Information
      Material Size10 μg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-352M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-352-D 04055977276541