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SCC255 WT-1 Mouse Brown Preadipocyte Cell Line

WT-1 mouse brown preadipocyte cell line is a useful model for studying adipogenesis and brown adipocyte biology.

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SCC255
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC255
      DescriptionWT-1 Mouse Brown Preadipocyte Cell Line
      OverviewObesity is a growing worldwide health epidemic that has spurred research into new avenues for treatment and control of metabolic disorders. Brown fat, a specialized adipose tissue characterized by increased energy expenditure, has emerged as an area of interest because manipulation of this tissue has potential to protect against diet-induced obesity by altering the balance of energy intake and consumption (1).

      The WT-1 mouse immortalized brown preadipocyte cell line is derived from primary brown preadipocytes. WT-1 preadipocytes can be induced to differentiate into brown adipocytes upon treatment with induction and differentiation factors. WT-1 cells express brown fat marker uncoupling protein-1 (UCP1) (3) and are a valuable model for investigation into mitochondrial physiology during adipogenesis. The WT-1 cell line has been used to develop 3D assays for adipose tissue (4) and is well suited for a range of questions in cellular metabolic research.

      Source:
      WT-1 cell line was established from primary brown pre-adipocytes isolated from newborn mouse pups and immortalized with Simian virus 40 (SV40) large T antigen (2).

      References:
      1. J Biomed Res. 2016 31(1): 1-2.
      2. PLoS One. 2009 4(12): e8458.
      3. Nature. 2008 454(7207): 1000-4.
      4. Biomaterials. 2016 75: 123-134.
      References
      Product Information
      Applications
      ApplicationWT-1 mouse brown preadipocyte cell line is a useful model for studying adipogenesis and brown adipocyte biology.
      Key Applications
      • Cell Culture
      • Cell Differentiation
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Adipocytes
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC255 04061841079828