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GF102 TWEAK, human

Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.

TWEAK, human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: O43508 Entrez Gene Number: NM_003809.2
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GF102
25 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesSourcePurity
      CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analyses
      Description
      Catalogue NumberGF102
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTWEAK, human
      OverviewHuman TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. GF102 is a 17.0 kDa protein, comprising the TNF-like extracellular domain of TWEAK.
      References
      Product Information
      PresentationSterile filtered and then the protein was lyophilized from 10 mM NaP pH 7.5 + 0.1 M L-Arg.
      Quality LevelMQ100
      Applications
      ApplicationHuman TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis.
      Key Applications
      • Cell Culture
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analyses
      SourceE.coli
      Specific ActivityBIOLOGICAL ACTIVITY: <br /><br />GF102 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC is less than 10ng/ml. <br /><br />GF102 weakly induces the death of HT29 cells when cultured in the presence of IFN-g. The ED50 for this effect is between 30-45 ng/ml.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.
      Gene Symbol
      • TNFSF12
      • MGC129581
      • DR3LG
      • TWEAK
      • APO3L
      • MGC20669
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43508 # Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
      SIZE: 249 amino acids; 27216 Da
      SUBUNIT: Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor ligand superfamily member 12, secreted form: Secreted.
      TISSUE SPECIFICITY: Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
      PTM: The soluble form derives from the membrane form by proteolytic processing.
      SIMILARITY: SwissProt: O43508 ## Belongs to the tumor necrosis factor family.
      Stem Cell Type
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Freeze/thaw cycles will result in significant loss of activity. After a quick spin, reconstitute in 10 mM NaP pH 7.5 to a concentration of 1 mg/ml. This solution can be diluted into other buffer solutions or stored at -20°C. Reconstituted TWEAK should be stored at -20°C or lyophilized in working aliquots.
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GF102 04053252511233

      Documentation

      TWEAK, human SDS

      Title

      Safety Data Sheet (SDS) 

      TWEAK, human Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN TWEAK 2933274
      RECOMBINANT HUMAN TWEAK 2898475
      RECOMBINANT HUMAN TWEAK 3774189
      RECOMBINANT HUMAN TWEAK 2898473
      RECOMBINANT HUMAN TWEAK - 2388301 2388301
      RECOMBINANT HUMAN TWEAK - 2051904 2051904
      RECOMBINANT HUMAN TWEAK - 2080854 2080854
      RECOMBINANT HUMAN TWEAK - 2089425 2089425
      RECOMBINANT HUMAN TWEAK - 2231598 2231598
      RECOMBINANT HUMAN TWEAK - 2565767 2565767
      Show more

      Data Sheet

      Title
      RECOMBINANT HUMAN TWEAK

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors