Millipore Sigma Vibrant Logo

69215 S•Tag™ Grade S-protein

S•Tag™ Grade S-protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69215
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Fulfillment and Delivery Delayed Fulfillment and Delivery Delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The FRETWorks™ S•Tag™ Assay is a FRET-based method that enables extremely sensitive detection of S•Tag fusion proteins in minutes with a homogeneous format. The interaction of the 15–amino acid S•Tag fusion peptide with purified S-protein reconstitutes RNase activity, which is measured using the FRET ArUAA substrate. The substrate consists of a short, mixed ribo/deoxyribooligonucleotide having a fluorophore on the 5′-end and a quencher on the 3′-end (Keleman 1999). Fluorescence of the uncleaved molecule is inhibited by the interaction of the fluorophore and quencher; upon cleavage of the intervening ribonucleotide residue, the fluorophore is released and becomes highly fluorescent. The specificity of the substrate permits this assay to be performed with crude extracts. No reagent injection is necessary, and the signal is stable for > 6 hours.

      The FRETWorks S•Tag Assay Kit is compatible with the PopCulture® Reagent (including the additives Benzonase® Nuclease and rLysozyme™ Solution), making these reagents an ideal combination for rapid, automatable screening of protein expression.
      Catalogue Number69215
      Brand Family Novagen®
      References
      References1. Kim, J.-S. and Raines, R.T. (1993) Protein Sci. 2, 348–356.

      2. McCormick, M. and Mierendorf, R. (1994) inNovations 1, 4–7.

      3. Richards, F.M. and Wyckoff, H.W. (1971) in The Enzymes, Vol. IV (Boyer, P.D., Ed.), p. 647–806, Academic Press, New York.
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69215 0

      Documentation

      S•Tag™ Grade S-protein Certificates of Analysis

      TitleLot Number
      69215

      References

      Reference overview
      1. Kim, J.-S. and Raines, R.T. (1993) Protein Sci. 2, 348–356.

      2. McCormick, M. and Mierendorf, R. (1994) inNovations 1, 4–7.

      3. Richards, F.M. and Wyckoff, H.W. (1971) in The Enzymes, Vol. IV (Boyer, P.D., Ed.), p. 647–806, Academic Press, New York.