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HTS137RTA Ready-to-Assay™ CaS Calcium Sensor Receptor Frozen Cells

Human CaS GPCR frozen cells for Calcium Flux FLIPR Assays.

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Host Cell: Chem-1 Entrez Gene Number: NM_000388
HTS137RTA
1 kit  
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      Overview

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1CaS
      Description
      Catalogue NumberHTS137RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CaS Calcium Sensor Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Calcium homeostasis in vertebrates is regulated by hormonal communication between the parathyroid and thyroid glands, kidney, bone, and intestine. A central mediator of this regulatory system is the calcium sensor CaS (also known as CaR), a class III GPCR that is activated by high concentrations of extracellular calcium and other divalent cations. The cell types that are responsive to variations in blood calcium concentration express CaS and respond to changes in extracellular calcium in a CaS-dependent fashion. Activation of CaS in parathyroid chief cells inhibits PTH release, which results in decreased calcium mobilization from bone. In contrast, stimulation of CaS activity in the C cells of the thyroid increases secretion of calcitonin, which causes decreased bone resorption and increased urinary excretion of calcium. Allosteric "calcimimetic" potentiators are being evaluated for treatment of primary hyperparathyroidism (Brown and MacLeod, 2001). Millipore’s cloned human CaS-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CaS expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CaS Receptor.
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman CaS GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassC
      Host CellsChem-1
      Protein TargetCaS
      Target Sub-FamilyCalcium sensor
      Cell Line Type
      • GPCR Cell Lines
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size1 kit
      Global Trade Item Number
      Catalogue Number GTIN
      HTS137RTA 04053252481314