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676602 Sigma-AldrichPhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

This PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: Anti-Vasodilator-stimulated Phosphoprotein, Activated

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676602

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Overview

Replacement Information

Key Spec Table

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

Description
Overview	Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.
Catalogue Number	676602
Brand Family	Calbiochem®
Synonyms	Anti-Vasodilator-stimulated Phosphoprotein, Activated
Application Data	Detection of human VASP, phosphorylated on Ser²³⁹, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.

References
References	Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168. Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145. Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 2X PBS, PEG, sucrose.
Positive control	Vanadate-treated A431 cells or 8-pCPT-treated platelets
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	ELISA (0.05 µg/ml) Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Comments	Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton^® X-100 detergent, and 0.05% Tween^®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
Immunogen	Human
Clone	16C2
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
676602	0

Documentation

PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) SDS

Title
Safety Data Sheet (SDS)

PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) Certificates of Analysis

Title	Lot Number
676602	Enter Lot Number

References

Reference overview
Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168. Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145. Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	16-August-2007 RFH
Synonyms	Anti-Vasodilator-stimulated Phosphoprotein, Activated
Application	ELISA (0.05 µg/ml) Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric) Immunocytochemistry (1-10 µg/ml) Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Data	Detection of human VASP, phosphorylated on Ser²³⁹, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.
Description	Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.
Background	VASP is a proline-rich substrate of PKA and PKG. It is involved in the enhancement of actin filament formation and has been shown to bind profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. VASP is expressed in most mammalian tissues, with particularly high expression in platelets.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
Clone	16C2
Isotype	IgG₁
Species	human, mouse
Positive control	Vanadate-treated A431 cells or 8-pCPT-treated platelets
Form	Lyophilized
Formulation	Lyophilized from 2X PBS, PEG, sucrose.
Preservative	≤0.1% sodium azide
Comments	Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton^® X-100 detergent, and 0.05% Tween^®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
Storage	-20°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168. Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145. Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.

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