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PS1024 PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10)

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PS1024
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~60 kDa Smad1/5 protein phosphorylated at Ser463/465 in HeLa cells treated with BMP-4.
      Catalogue NumberPS1024
      Brand Family Calbiochem®
      Application Data
      Detection of human Smad1/5, phosphorylated at pSer463/465, by immunoblotting. Samples: whole cell lysates from HeLa or NIH/3T3 cells, left untreated or treated with BMP-4, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:1000). Detection: chemiluminescence.

      Detection of human Smad1/5 phosphorylated at pSer463/465, by flow cytomtry. Samples: HeLa cells left untreated (blue) or treated with BMP-4 (green). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:200) (blue and green) and a nonspecific, negative control antibody (red). Detection: fluorescence.
      References
      ReferencesKlemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
      Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
      Hoodless, P.A., et al. 1996. Cell 296, 1646.
      Product Information
      FormLiquid
      FormulationIn 50% glycerol, 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, pH 7.5.
      Preservative≤0.1 sodium azide
      Applications
      Key Applications Flow Cytometry
      Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Flow Cytometry (1:200)
      Application CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of a secondary antibody. Antibody should be titrated for optimal results in individual sytems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 463/465 phosphorylation sites of human Smad5, coupled to KLH.
      ImmunogenHuman
      Clone41D10
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PS1024 0

      Documentation

      PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) Certificates of Analysis

      TitleLot Number
      PS1024

      References

      Reference overview
      Klemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
      Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
      Hoodless, P.A., et al. 1996. Cell 296, 1646.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-May-2009 JSW
      ApplicationImmunoblotting (1:1000)
      Flow Cytometry (1:200)
      Application Data
      Detection of human Smad1/5, phosphorylated at pSer463/465, by immunoblotting. Samples: whole cell lysates from HeLa or NIH/3T3 cells, left untreated or treated with BMP-4, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:1000). Detection: chemiluminescence.

      Detection of human Smad1/5 phosphorylated at pSer463/465, by flow cytomtry. Samples: HeLa cells left untreated (blue) or treated with BMP-4 (green). Primary antibody: PhosphoDetect™ Anti-Smad1/5 (pSer463/465) Rabbit mAb (41D10) (Cat. No. PS1024) (1:200) (blue and green) and a nonspecific, negative control antibody (red). Detection: fluorescence.
      DescriptionPurified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~60 kDa Smad1/5 protein phosphorylated at Ser463/465.
      BackgroundBone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis. BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors. Subsequently, the activated receptor phosphorylate Smad1 at Ser463 and Ser465 in the C-terminal motif, SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with Smad4 and translocate to the nucleus, where the transcription of target genes is stimulated.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 463/465 phosphorylation sites of human Smad5, coupled to KLH.
      Clone41D10
      IsotypeIgG
      Specieshuman, mouse
      FormLiquid
      FormulationIn 50% glycerol, 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, pH 7.5.
      Preservative≤0.1 sodium azide
      CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of a secondary antibody. Antibody should be titrated for optimal results in individual sytems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Toxicity Standard Handling
      ReferencesKlemm, J.D., et al. 1998. Annu. Rev. Immunol. 16, 569.
      Kretzschumar, M., et al. 1997. Genes Dev. 11, 984.
      Hoodless, P.A., et al. 1996. Cell 296, 1646.