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PS1014 PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb

This PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK2 (pTyr1007/1008).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008

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PS1014
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells.
      Catalogue NumberPS1014
      Brand Family Calbiochem®
      SynonymsAnti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008
      Application Data
      Detection of mouse JAK2, phosphorylated at Tyr1007/1008, by immunoblotting. Samples: Whole cell lysate from NIH3T3-L1 cells left unstimulated (lane 1) or stimulated with leukemia inhibitor factor (LIF) (lanes 2-4). Primary antibody: PhosphoDetect™ Anti-JAK2 (Tyr1007/1008) Rabbit pAb (Cat. No. PS1014) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
      References
      ReferencesFrank, G.D., et al. 2003. Mol. Biol. 23, 1581.
      Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
      Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
      Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
      Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
      Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
      Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
      Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
      Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
      Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
      Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
      Ihle, J.N., et al. 1996. Cell 84, 331.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
      Positive control3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PS1014 0

      Documentation

      PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-JAK2 (pTyr1007/1008) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PS1014

      References

      Reference overview
      Frank, G.D., et al. 2003. Mol. Biol. 23, 1581.
      Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
      Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
      Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
      Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
      Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
      Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
      Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
      Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
      Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
      Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
      Ihle, J.N., et al. 1996. Cell 84, 331.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-October-2007 RFH
      SynonymsAnti-Janus Kinase 2, Phospho-Specific (Tyr1007/1008
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of mouse JAK2, phosphorylated at Tyr1007/1008, by immunoblotting. Samples: Whole cell lysate from NIH3T3-L1 cells left unstimulated (lane 1) or stimulated with leukemia inhibitor factor (LIF) (lanes 2-4). Primary antibody: PhosphoDetect™ Anti-JAK2 (Tyr1007/1008) Rabbit pAb (Cat. No. PS1014) (1:1000) pre-incubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), or the phosphopeptide immunogen (lane 4). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK2 protein.
      BackgroundJanus Kinase 2 (JAK2) belongs the the JAK family of non-receptor tyrosine kinases. The JAK family consists of four members, JAK1, JAK2, JAK3, and TYK2. Ligand binding to a variety of cell surface receptors, like cytokine and growth factor receptors, results in the association and subsequent activtion of JAK kinases via phosphorylation. Activated JAK proteins, in turn, phosphorylate members of the Signal Transducers and Activators of Transcription (STAT) family of transcription factors. In the JAK2 domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor and contains two tyrosines, Tyr1007 and Tyr1008, that are key phoshorylation/regulatory sites.
      HostRabbit
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive control3T3-L1 adipocytes stimulated with leukemia inhibitor factor (LIF)
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (± 0.1).
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesFrank, G.D., et al. 2003. Mol. Biol. 23, 1581.
      Kaszubska, W., et al. 2002. Mol. Cell. Endocrinol. 195, 109.
      Kirito, K., et al. 2002. J. Biol. Chem. 277, 8329.
      Shaw, S., et al. 2002. J. Biol. Chem. 277, 44920.
      Sutherland, C.L., et al. 2002. J. Immunol. 168, 671.
      Zeng, Z.Z., et al. 2002. J. Biol. Chem. 277, 41213.
      Eguchi, S., et al. 2001. J. Biol. Chem. 276, 7957.
      Madamanchi, N.R., et al. 2001. J. Biol. Chem. 276, 18915.
      Myers, M.P., et al. 2001. J. Biol. Chem. 276, 47771.
      Madamanchi, N.R., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 21, 321.
      Amiri, F., et al. 1999. J. Biol. Chem. 274, 32382.
      Ihle, J.N., et al. 1996. Cell 84, 331.