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524390
Sigma-AldrichPhorbol-12,13-dibutyrate - CAS 37558-16-0 - Calbiochem
Strong irritant for mouse skin, but only moderately active as a tumor promoter.
More>>Strong irritant for mouse skin, but only moderately active as a tumor promoter. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C. Stimulates the phosphorylation of Na+,K+- ATPase, thereby inhibiting its activity. Promotes the expression of inducible NOS in cultured hepatocytes. Commonly used in binding studies or in applications requiring high concentrations of phorbol compounds.
Catalogue Number
524390
Brand Family
Calbiochem®
Synonyms
PDBu, PKC Activator II
References
References
Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427. Lee, E.J., et al. 1994. Brain Res. 665, 127. Critz, S.D., et al. 1993. J. Neurochem.60, 1175. Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611. Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079. Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937. Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Very toxic by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Limited evidence of a carcinogenic effect. Risk of serious damage to eyes.
S Phrase
S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic & Carcinogenic / Teratogenic
Hazardous Materials Attention:
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
Packaging Information
Packaged under inert gas
Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number
GTIN
524390-1MG
04055977271546
524390-5MG
04055977271553
Documentation
Phorbol-12,13-dibutyrate - CAS 37558-16-0 - Calbiochem SDS
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-August-2008 RFH
Synonyms
PDBu, PKC Activator II
Description
Strong irritant for mouse skin, but only moderately active as a tumor promoter. Activates protein kinase C (PKC). Stimulates the phosphorylation of Na+-K+ ATPase, thereby inhibiting its activity. Promotes the expression of iNOS in cultured hepatocytes. PDBu can be diluted to 30 µM in aqueous solutions, so it is commonly used in binding studies or in applications requiring high concentrations. It is easier to remove PDBu than PMA from cells in culture.
Form
Clear film or white crystalline solid
Intert gas (Yes/No)
Packaged under inert gas
CAS number
37558-16-0
RTECS
EK7767000
Chemical formula
C₂₈H₄₀O₈
Structure formula
Purity
≥99% by HPLC
Solubility
DMSO (10 mg/ml). Further dilute with aqueous buffers just prior to use.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
Toxicity
Highly Toxic & Carcinogenic / Teratogenic
References
Fisone, G., et al. 1995. J. Biol. Chem. 270, 2427. Lee, E.J., et al. 1994. Brain Res. 665, 127. Critz, S.D., et al. 1993. J. Neurochem.60, 1175. Iredale, P.A., et al. 1993. Biochem. Pharmacol.45, 611. Critz, S.D., and Byrne J.H. 1992. J. Neurophys.68, 1079. Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937. Ward C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
