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109254
Sigma-AldrichPapanicolaou's solution 1b Hematoxylin solution S
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Description
Catalogue Number
109254
Description
Papanicolaou’s solution 1b Hematoxylin solution S
Overview
Papanicolaou’s solution 1b Hematoxylin solution S - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65,(Cat.no. 109270), or Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Both Papanicolaou’s solution 1a Harris hematoxylin solution(Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S (Cat no.109254) give a blue to dark violet staining result with clinical specimen material. A 500 ml bottle of either solution is sufficient for 1500 -2500 slides. It is registered as IVD and CE certified product and can be used for clinical diagnostics. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
H302: Harmful if swallowed. H319: Causes serious eye irritation. H373: May cause damage to organs through prolonged or repeated exposure if swallowed.
Precautionary Statement(s)
P260: Do not breathe mist or vapours. P264: Wash skin thoroughly after handling. P280: Wear eye protection/ face protection. P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P314: Get medical advice/ attention if you feel unwell.
Signal Word
Warning
Storage class
10 - 13 Other liquids and solids
WGK
WGK 1 slightly hazardous to water
Disposal
3 Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of danger
harmful
Product Usage Statements
Storage and Shipping Information
Storage
Store at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)
passes test
Nuclei
blue to dark violet
Cyanophilic Cytoplasm (basophilic)
blue-green
Eosinophilic Cytoplasm (acidophilic)
pink
Global Trade Item Number
Catalogue Number
GTIN
1092540500
04022536112510
1092542500
04022536112534
Documentation
Papanicolaou's solution 1b Hematoxylin solution S SDS
