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14-906M PI3K-C2α Protein, 250 µg

N-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end. For use in Kinase Assays.

PI3K-C2α Protein, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-906M
250 µg  Also available in 10 μg size (cat#14-906) and in bulk (cat# 14-906-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-906M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPI3K-C2α Protein, 250 µg
      OverviewN-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end.
      References
      Product Information
      Presentation10 μg of enzyme in 10.6 μl of 50 mM Tris/HCl, pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol, 20 mM β-glycerophosphate, 0.25 mM Na3VO4, 10 mM NaF. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationN-terminal 6His-tagged recombinant human PI3K-C2α amino acids 299–end. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.
      Gene Symbol
      • PI3-kinase
      • PIK3
      • PI3K
      • PTDINS-3-kinase
      • PI3K C2a
      • PI3-Kinase Class II
      Protein TargetPI3K-C2α
      Purification MethodImmobilized metal affinity chromatography (IMAC)
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2. May play a role in clathrin-coated endocytic vesicle formation and EGF signaling cascade. May be involved in mitosis and UV-induced damage response. May be a downstream effector in insulin signaling cascade.
      CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.
      COFACTOR: Calcium or magnesium. Manganese cannot be used.
      ENZYME REGULATION: Activated by insulin. Only slightly inhibited by wortmannin and LY294002. Activated by clathrin.
      Subunit structure: Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers.
      SUBCELLULAR LOCATION: Cell membrane. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Nucleus. Cytoplasm. Associated with RNA-containing structures.
      TISSUE SPECIFICITY: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney.
      SIMILARITY: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain. Contains 1 PX (phox homology) domain.
      Molecular Weight161kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in the PIProfiler.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-906) and in bulk (cat# 14-906-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-906M 04053252283680

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-906 PI3K-C2α Protein, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases