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521220 PDGF, AB Heterodimer, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhPDGF-AB

521220
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥97% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Recombinant, human platelet-drived growth factor AB (produced by the in vitro dimerization of PDGF-A and PDGF-B monomers.) and expressed in E. coli.
      Catalogue Number521220
      Brand Family Calbiochem®
      SynonymsrhPDGF-AB
      References
      ReferencesHannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
      Williams, L.T. 1989. Science 243, 1564.
      Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods enzym. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered 40% acetonitrile, 0.1% TFA.
      Applications
      Biological Information
      Biological activityED₅₀ = 1-3 ng/ml as measured by stimulation of ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg PDGF-AB
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      521220 0

      Documentation

      PDGF, AB Heterodimer, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      521220

      References

      Reference overview
      Hannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
      Williams, L.T. 1989. Science 243, 1564.
      Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods enzym. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-May-2008 RFH
      SynonymsrhPDGF-AB
      DescriptionRecombinant, human platelet-drived growth factor AB (produced by the in vitro dimerization of PDGF-A and PDGF-B monomers.) and expressed in E. coli.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 40% acetonitrile, 0.1% TFA.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg PDGF-AB
      Biological activityED₅₀ = 1-3 ng/ml as measured by stimulation of ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 7525
      ReferencesHannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
      Williams, L.T. 1989. Science 243, 1564.
      Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
      Betsholtz, C., et al. 1986. Nature 320, 695.
      Raines, E.W., et al. 1985. Methods enzym. 109, 749.
      Johnson, A., et al. 1984. EMBO J. 3, 921.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors