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71378 NoShift™ Transcription Factor Assay Kit Plus NucBuster™

An easy and rapid alternative to EMSA

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71378
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The NoShift™ Transcription Factor Assay Kit Plus NucBuster™ incorporates the NoShift™ Transcription Factor Assay Kit (Cat. No. 71377) with NucBuster™ Protein Extraction Reagent for convenient production of nuclear protein extracts. The NoShift™ Transcription Factor Assay Kit is a microassay plate–based method for analysis of DNA-binding proteins that alleviates many of the problems associated with gel shift assays. The gel shift, or electrophoretic mobility shift assay (EMSA), is a method for analyzing interactions between proteins and DNA. This technique, first published in 1981 (Fried 1981, Garner 1981), is based on the reduced electrophoretic mobility of protein–DNA complexes compared with that of DNA alone under nondenaturing conditions. The traditional gel shift assay is tedious and time consuming, requires radio-labeled probe, and often requires extensive optimization. The 96-well NoShift plate format is versatile and enables simultaneous analysis of multiple binding factors in less than five hours without the use of radioisotopes. A biotinylated oligonucleotide is mixed with a protein sample and the protein–DNA complex is captured on streptavidin plates. The bound transcription factor is detected with a specific antibody followed by a second antibody–HRP conjugate and chromogenic substrate.

      Each NoShift Transcription Factor Assay Kit comes with one 96-strip-well streptavidin-coated plate, binding reaction buffer, antibody dilution buffer, plate wash buffer, and TMB substrate and provides enough reagents for 100 reactions. Also available is the NoShift Transcription Factor Assay Kit Plus Nucbuster™ which includes a NucBuster™ Protein Extraction Kit developed for rapid extraction of nuclear protein. The NoShift Kit is designed for use with oligonucleotides defining a protein binding consensus sequence provided by the purchaser and an antibody to a DNA binding protein.
      Catalogue Number71378
      Brand Family Novagen®
      References
      References

      Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.
      Product Information
      •4 × 1 ml4X NoShift™ Bind Buffer
      •150 µlSalmon Sperm DNA
      •125 µlPoly(dI:dC):Poly(dI:dC)
      •30 ml10X NoShift Wash Buffer
      •20 mlNoShift Antibody Dilution Buffer
      •1 eaStrepavidin Plate
      •12 mlTMB Substrate
      •100 µl100 mM DTT
      •3 eaAluminum Plate Sealer
      •1 kitNucBuster™ Protein Extraction Reagent
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71378 0

      Documentation

      NoShift™ Transcription Factor Assay Kit Plus NucBuster™ Certificates of Analysis

      TitleLot Number
      71378

      References

      Reference overview

      Fried, M. and Crothers, D.M. 1981. Nucleic Acids Res. 9, 6505. Garner, M.M. and Revzin, A. 1981. Nucleic Acids Res. 9, 3047.

      Brochure

      Title
      Kit SourceBook - 2nd Edition EURO

      Citations

      Title
    • Markus Bredel, et al. (2006) Tumor necrosis factor-α-induced protein 3 as a putative regulator of nuclear factor-κB-mediated resistance to O6-alkylating agents in human glioblastomas. Journal of Clinical Oncology 24, 274-287.
    • Zachary T. Resch, et al. (2005) Stress-activated signaling pathways mediate the stimulation of pregnancy-associated plasma protein-A expression in cultured human fibroblasts. Endocrinology 147, 885-890.
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      User Protocols

      Title
      TB403 NoShift™ Transcription Factor Assay Kit