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481909 Niclosamide - CAS 50-65-7 - Calbiochem

A salicylanilide anthelmintic drug that acts as a potent and selective Stat3- and Wnt-signaling pathway inhibitor.

Niclosamide - CAS 50-65-7 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX

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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      50-65-7C₁₃H₈Cl₂N₂O₄
      Description
      OverviewA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212). Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 and Dishevelled-2 downregulation. Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa reporter assays). Efficiently inhibits breast cancer stem-like cells in vitro and in vivo.
      Catalogue Number481909
      Brand Family Calbiochem®
      Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX
      References
      ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
      Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
      Gies, E., et al. 2010. PLoS ONE 5, e14410.
      Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
      Chen, M., et al. 2009. Biochemistry 48, 10267.
      Product Information
      CAS number50-65-7
      FormPale yellow solid
      Hill FormulaC₁₃H₈Cl₂N₂O₄
      Chemical formulaC₁₃H₈Cl₂N₂O₄
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Regulatory Review
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      481909 0

      Documentation

      Niclosamide - CAS 50-65-7 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Niclosamide - CAS 50-65-7 - Calbiochem Certificates of Analysis

      TitleLot Number
      481909

      References

      Reference overview
      Wang, Y.C., et al. 2013. PLoS ONE 8, e74538.
      Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
      Gies, E., et al. 2010. PLoS ONE 5, e14410.
      Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
      Chen, M., et al. 2009. Biochemistry 48, 10267.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-March-2012 JSW
      Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX
      DescriptionA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy and use in tapeworm treatment, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212), causing MCF-7 autophagy activation under nutrient-rich conditions (by 15-fold with 4h 10 µM treatment), SH-SY5Y lysosome redistribution (10 µM for 8 h), and dissipation/clearance of ubiquinated protein aggregates upon proteasome inhibition in a reversible, time- and dose-dependent manner. Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 endocytosis (t1/2 = 2.4 h; effective conc. <2 µM) and Dishevelled-2 downregulation (IC50 ~1 µM), effectively preventing Wnt3a from stimulating HEK293 cellular LEF/TCF transcription activity (IC50 = 0.5 µM TOPFlash reporter assays). Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa-based pLucTKS3 reporter assays) by suppressing the phosphorylation of Jak1 Tyr1022/1023 (2 h treatment at 0.5 to 2.0 µM in Du145 cultures) and Stat3 Tyr705 (by >90% after 24 h treatment at 2 and 5 µM, respectively, in Du145 and HeLa cultures), without affecting the phosphorylation of Stat1 Tyr701, Stat3 Ser727, Stat5 Tyr694, or Jak2 Tyr1007/1008.
      FormPale yellow solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number50-65-7
      Chemical formulaC₁₃H₈Cl₂N₂O₄
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (2.5 mg/ml) or Ethanol (5 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Regulatory Review
      ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
      Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
      Gies, E., et al. 2010. PLoS ONE 5, e14410.
      Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
      Chen, M., et al. 2009. Biochemistry 48, 10267.