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444213 MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase

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444213
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444213-5UG
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      Description
      OverviewNative pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.
      Note: 1 mU = 1 milliunit.
      Catalogue Number444213
      Brand Family Calbiochem®
      SynonymsMatrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
      References
      ReferencesBuisson-Leqendre, N., et al. 2004. Arth. Rheumat. 50, 2151.
      Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7414.
      Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
      EC number3.4.24.24
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity≥600 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Contaminantsno other MMP activity detectable
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      444213-5UG 04055977205190