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444146 MDM2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mouse Double Minute 2, Human, Recombinant, E. coli

444146
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      We apologize for the inconvenience, but we do not currently have an alternative product. For related products, please refer to our complete listing of proteasome and ubiquitination enzymes.





      Recombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
      Catalogue Number444146
      Brand Family Calbiochem®
      SynonymsMouse Double Minute 2, Human, Recombinant, E. coli
      References
      ReferencesBond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
      Dornan, D., et al. 2004. Nature 429, 86.
      Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
      Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
      Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
      Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J. D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J. D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
      Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.
      Product Information
      Unit of DefinitionOne unit is defined as 1 ng purified protein.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Applications
      Biological Information
      Biological activity20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay.
      Purity> 95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444146 0

      Documentation

      MDM2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      444146

      References

      Reference overview
      Bond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
      Dornan, D., et al. 2004. Nature 429, 86.
      Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
      Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
      Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
      Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J. D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J. D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
      Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-November-2007 JSW
      SynonymsMouse Double Minute 2, Human, Recombinant, E. coli
      DescriptionRecombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity> 95% by SDS-PAGE
      Biological activity20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay.
      Unit definitionOne unit is defined as 1 ng purified protein.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initital thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBond G.L., et al. 2005 Curr. Cancer Drug Targets 5, 3.
      Dornan, D., et al. 2004. Nature 429, 86.
      Chen, J., et al., 1996. Mol. Cell. Biol. 16, 2445.
      Gorgoulis, V.G., et al. 1996. J. Pathol. 180, 129.
      Finlay, C. A. 1993. Mol. Cell. Biol. 13, 301.
      Leach, F. S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J. D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J. D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S. et al. 1991. EMBO J. 10, 1565.
      Cahilly-Snyder, L., et al. 1987. Somat. Cell Mol. Genet. 13, 235.