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429705 Leptin, Mouse, Recombinant, E. coli

Leptin, Mouse, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: βrmOB

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429705
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca2+-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis.
      Catalogue Number429705
      Brand Family Calbiochem®
      SynonymsβrmOB
      References
      ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
      Ookuma, M., et al. 1998. Diabetes 47, 219.
      Campfield, L.A., et al. 1995. Science 269, 546.
      Halaas, J.L., et al. 1995. Science 269, 543.
      Pelleymounter, M.A., et al. 1995. Science 269, 540.
      Zhang, Y., et al. 1994. Nature 372, 425.
      Product Information
      CAS number181030-10-4
      FormLyophilized
      FormulationLyophilized from a sterile filtered PBS.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg leptin
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      429705 0

      Documentation

      Leptin, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Leptin, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      429705

      References

      Reference overview
      Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
      Ookuma, M., et al. 1998. Diabetes 47, 219.
      Campfield, L.A., et al. 1995. Science 269, 546.
      Halaas, J.L., et al. 1995. Science 269, 543.
      Pelleymounter, M.A., et al. 1995. Science 269, 540.
      Zhang, Y., et al. 1994. Nature 372, 425.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-March-2009 JSW
      SynonymsβrmOB
      DescriptionRecombinant, mouse leptin espressed in E. coli. Leptin was originally identified as a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares ~84% sequence identity with the mouse protein. Human leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid signal sequence that is cleaved to yield the 146 amino acid mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue.

      A high-affinity receptor for leptin (OB-R) with homology to gp130 and the G-CSF receptor was subsequently cloned. The OB-R cytoplasmic domain transduces the leptin signal through the JAK-STAT pathway. Although OB-R mRNA was initially shown to be expressed predominantly in the choroid plexus and in the hypothalamus, more recent data also revealed the expression of this receptor in endothelial cells (Ecs). Furthermore, the angiogenic activity of leptin has been demonstrated both in vitro and in vivo, suggesting a physical mechanism whereby leptin-induced angiogenesis may facilitate increased energy expenditure.
      FormLyophilized
      FormulationLyophilized from a sterile filtered PBS.
      CAS number181030-10-4
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg leptin
      Biological activityED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
      SolubilityReconstitute with 20 mM Tris-HCl, pH 8.0 to a final concentration of 1 mg/ml.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      Merck USA index14, 5443
      ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
      Ookuma, M., et al. 1998. Diabetes 47, 219.
      Campfield, L.A., et al. 1995. Science 269, 546.
      Halaas, J.L., et al. 1995. Science 269, 543.
      Pelleymounter, M.A., et al. 1995. Science 269, 540.
      Zhang, Y., et al. 1994. Nature 372, 425.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Proteins