Millipore Sigma Vibrant Logo

EZGRT-89BK Human Ghrelin (total) ELISA- Bulk

This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.

Human Ghrelin (total) ELISA- Bulk MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
View Products on Sigmaaldrich.com
EZGRT-89BK
Ten 96-well strip plates  This product consists of ten Human Ghrelin (total) ELISA kits.
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Fulfillment and Delivery Delayed Fulfillment and Delivery Delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (Total) HELISAFluorescent Colorimetric
      Description
      Catalogue NumberEZGRT-89BK
      DescriptionHuman Ghrelin (total) ELISA- Bulk
      Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
      • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
      Quality LevelMQ200
      Applications
      ApplicationThis Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      SpecificityHuman Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin (Total)
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • obestatin
      • MTLRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • Serum: (Active Ghrelin) 88–91%
      • (Des-octanoyl Ghrelin) 101–107%
      • Plasma: (Active Ghrelin) 84–87%
      • (Des-octanoyl Ghrelin) 112–119%
      Physicochemical Information
      Sensitivity
      • 156 pg/mL (20 µL sample size)
      Linearity of DilutionSerum: 95.7–104.7% Plasma: 95–117%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material SizeTen 96-well strip plates
      Material PackageThis product consists of ten Human Ghrelin (total) ELISA kits.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      EZGRT-89BK 04053252436901

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      EZGRA-88K Human Ghrelin (active) ELISA Show Pricing & Availability
      EZGRT-89K Human Ghrelin (total) ELISA Show Pricing & Availability
      EZRGRT-91K Rat/Mouse Ghrelin (total) ELISA Show Pricing & Availability
      HMHEMAG-34K MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA)