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14-623M HIPK2 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564, for use in Kinase Assays.

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14-623M
250 µg  Also available in 10 μg size (cat#14-623) and in bulk (cat#14-623-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-623M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHIPK2 Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, human HIPK2, amino acids 165-564, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourcebaculovirus in Sf21 insect cells
      Entrez Gene Number
      Entrez Gene SummaryHIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.[supplied by OMIM]
      Gene Symbol
      • HIPK2
      • PRO0593
      • hHIPk2
      Protein TargetHIPK2
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9H2X6 # Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity.
      SIZE: 1198 amino acids; 130966 Da
      SUBUNIT: Interacts with TRADD, TP53, TP73, TP63, CREBBP, DAXX, P53DINP1, SKI, SMAD1, SMAD2 and SMAD3, but not SMAD4. Interacts with NKX1-2, NKX2-5, SPN/CD43, UBE2I, HMGA1, CTBP1, AXIN1, NLK, MYB, POU4F1, POU4F2, POU4F3, UBE2I, UBL1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Interacts with CBX4.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.TISSUE SPECIFICITY: Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
      PTM: Phosphorylated on tyrosines (By similarity). Autophosphorylated. & Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
      SIMILARITY: SwissProt: Q9H2X6 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily. & Contains 1 protein kinase domain.
      Molecular Weight49.6kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 0.33mg/ml MBP (Catalog # 13-104)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-623) and in bulk (cat#14-623-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-623M 04053252924422

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-623 HIPK2 Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases