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71867 Gateway® Nova pCOLA-2-DEST Expression System

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71867
  
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      Overview

      Description
      Overview

      This product has been discontinued.





      The Gateway® Nova pCOLA-2-DEST™ vector carries the COLA replicon from ColA and the kanamycin resistance gene. This DEST vector is compatible with pENTR vectors created using pDONR223, the pDONR vector used in the human ORFeome project. This DEST vector is NOT compatible with pENTR vectors derived from pDONR221 or other pENTR vectors carrying the kanR gene on their backbone. pEXPR recombinants created using pCOLA-2-DEST can be used for coexpression with expression plasmids generated from Gateway Nova pET-DEST vectors carrying the ColE1 replicon and AmpR gene. pCOLA-2-DEST is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal Strep•Tag® II coding sequence. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation, recombination & expression, and the LR Clonase® II enzyme for the recombination reaction.
      Catalogue Number71867
      Brand Family Novagen®
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      71867 0