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70850 FastPlax™ Titer Kit - Novagen

Rapid, reliable titering of baculovirus stocks

FastPlax™ Titer Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70850
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70850-3
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      		 Plastic ampoule 5 ea
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      Description
      OverviewThe FastPlax™ Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

      The FastPlax Antibody is used to detect the appearance of AcNPV gp64 glycoprotein on the surface of infectted cells as early as 8-24 hours post-infection. This forms the basis of the FastPlax Titer Kit, which allows determination of baculovirus titers in just 25-48 hours.

      The FastPlax Antibody can also be used to detect gp64 on Western blots.
      Catalogue Number70850
      Brand Family Novagen®
      References
      Product Information
      •2 × 50 ml10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
      •6 ml10% Gelatin (contains 0.02% azide)
      •10 µlFastPlax Antibody
      •300 µlGoat-Anti-Mouse β-Galactosidase Conjugate
      •300 µlNBT (83 mg/ml in 70% dimethylformamide)
      •300 µlX-gal (50 mg/ml in dimethylformamide)
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70850-3 04055977272642