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L6135-BC Easycoll, Density 1.077 g/ml

L6135-BC
  
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      Overview

      Description
      OverviewThe basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).
      Specifications:

      Sterility: Pass
      Functional Test: Pass
      pH: 7.08-7.58
      Osmolality: 0.280-0.303 Osm/kg H20
      Density: 1.076-1.077 g/ml
      Color: Light Yellow
      Appearance: Opaque
      Separating solutions

      Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation.
      We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

      Easycoll

      The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).

      Production of Easycoll with different densities

      The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value.
      But you can also adjust a required density yourself by diluting Easycoll correspondingly.
      The table below shows an example for different densities. All amounts are given for a temperature of +20°C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions might influence agglomeration and hence separation of cell clusters.
      Tab. 37: Instruction how to dilute the stock solution of Easycoll (density 1.124 g/ml; cat. no. L6143/L6145) with PBS (cat. no. L1825/0)
      Preparation of solutions with other densities can be made by isoosmolar dilution according to the following formula:
      For the dilution we recommend the use of PBS without Ca2+, Mg2+, since both ions can influence the agglomeration of cells and consequently cell separation.
      Catalogue NumberL6135-BC
      Brand Family Calbiochem®
      Product Information
      Quality LevelMQ300
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      L6135-BC 0