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17-10388 Di-Ubiquitin (Lys63-linked) Recombinant Protein

Native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.

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17-10388
50 ug  
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      Overview

      Replacement Information
      Description
      Catalogue Number17-10388
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionDi-Ubiquitin (Lys63-linked) Recombinant Protein
      OverviewANTIBODY AVAILABLE!
      Anti-Ubiquitin, Lys63-Specific, clone Apu3 ( catalogue # 05-1308)

      This product is a native K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs).
      This product is formed by chemical ligation.
      Background InformationPolyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.

      In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
      References
      Product Information
      Components
      • 50 µg of Di-Ubiquitin (Lys63-linked) reconstituted in 2.5 µL DMSO and 56 µL of 0.02 M TRIS, 0.25 M NaCl, pH 7.5 to a final concentration of 50 µM.
      Quality LevelMQ100
      Applications
      ApplicationNative K63 linked Di-Ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.
      Application NotesLC-MS Analysis
      Mobile phase A = 1% CH3CN, 0.1% formic acid in water (MilliQ) and B = 1% water (milliQ) and 0.1% formic acid in
      CH3CN. Phenomenex Kinetex C18, (2.1×50 mm, 2.6 μM); flow rate = 0.6 mL/min, runtime = 6 min, column T = 40°C.
      Gradient: 5% to 95% B over 3.5 min. (See Figure 2)
      Biological Information
      Purity> 95% by RP-HPLC
      Gene Symbol
      • Ub
      • Ubiquitin
      Molecular Weight~17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      0.5-2 μg/mL of anti-Ubiquitin, Lys63-Specific, clone Apu2 (Cat. # 05-1308) detected Lys63-linked Ubiquitin from 1 µg of Di-Ubiquitin (Lys63-linked) Recombinant Protein (Cat. # 17-10388). (See Figure 1)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. Avoid freeze/thaw cycles.
      Packaging Information
      Material Size50 ug
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      17-10388 04053252914652