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322133 DSP, Water-Soluble

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322133
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Overview

Replacement Information

Key Spec Table

Purity
≥94% by HPLC

Pricing & Availability

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322133-100MG
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      Plastic ampoule 100 mg
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      Description
      OverviewBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.
      Catalogue Number322133
      Brand Family Calbiochem®
      Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
      References
      ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
      Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
      D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
      Yamada, H., et al. 1981. Biochem. 20, 4836.
      Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.
      Product Information
      FormOff-white to light tan solid
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥94% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      322133-100MG 04055977216318

      Documentation

      DSP, Water-Soluble SDS

      Title

      Safety Data Sheet (SDS) 

      DSP, Water-Soluble Certificates of Analysis

      TitleLot Number
      322133

      References

      Reference overview
      Howard, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
      Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
      D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
      Yamada, H., et al. 1981. Biochem. 20, 4836.
      Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
      DescriptionBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.
      FormOff-white to light tan solid
      Structure formulaStructure formula
      Purity≥94% by HPLC
      SolubilityH₂O (6 mg/ml)
      Storage Protect from moisture
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
      Toxicity Irritant
      ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
      Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
      D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
      Yamada, H., et al. 1981. Biochem. 20, 4836.
      Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.