EAMT001 Cytochrome c ELISA Kit

This product has been discontinued.

The permeabilization of the mitochondrial outer membrane and the consequent release of cytochrome c and other apoptogenic proteins from the mitochondrial intermembrane space into cytoplasm is considered hallmark of many apoptotic pathways leading to cell death. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is a prime interest of many researchers. Most of the current biochemical methods of quantification of cytoplasmic and mitochondrial cytochrome c involve mechanical disruption of cells to obtain mitochondria-enriched and cytosolic fractions. These methods are time-consuming and limited to small number of samples.

The Cytochrome C ELISA Kit is used to determine the amount of the cytochrome c in a sample. Cytochrome c is immunocaptured within the wells and the amount is determined by adding a cytochrome c specific antibody conjugated with horseradish peroxidase. This peroxidase changes the substrate from colorless to blue. This reaction takes place in a time dependent manner proportional to the amount of protein captured in the wells. The rate of development of blue color can be followed at 600 nm or the reaction can be stopped, at a user defined time, by the addition of 100 µL of 1.5 N HCl to each well (not supplied) and read at 450 nm.

15 ml 	20X Buffer, (72594-15ML)
15 ml	10X Blocking Solution, (72595-15ML)
15 ml	20X Wash Buffer, (72596-15ML)
20 ml	HRP Development Solution, (72597-10ML)
1 ml	Detergent, (72598-1ML)
1 ml	Detector Antibody, (72599-1ML)
1 ml	20X HRP label, (72600-1ML)
1 ea	Microplate, (72601-1EA)

* This kit contains sufficient materials for 96 measurements (one 96-well microplate).

Storage: Store all components at 4°C.

This kit contains the necessary buffers, detergent for sample preparation, and substrate (HRP Development Solution) for the respective reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.

The Cytochrome C ELISA Kit contains sufficient materials for 96 measurements (one 96-well microplate).