HTS142M
Sigma-AldrichChemiScreen™ Membrane Preparation
Recombinant Human EP4 Prostanoid Receptor
Human EP4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
More>> Human EP4 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. Less<<Recommended Products
Overview
Key Spec Table
Species | Host Cells | Protein Target |
---|---|---|
Human | Chem-1 | EP4 |
Product Information | |
---|---|
Format | Membranes |
Presentation | One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H-labeled prostaglandin E2 at 1.5 nM. Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. |
Quality Level | MQ100 |
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of EP4 membrane prep:
Product Usage Statements |
---|
10 µg/well | |
---|---|
Signal:Background | 3.9 |
Specific Binding (cpm) | 643 |
1 unit = 10 µg membrane preparation
Bmax: 16.1 pmol/mg
Kd: 1.57 nM Usage Statement
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information | |
---|---|
Storage Conditions | Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. |
Packaging Information | |
---|---|
Material Size | 200 units |
Global Trade Item Number | |
---|---|
Catalogue Number | GTIN |
HTS142M | 04053252325236 |