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219497 Cathepsin L Substrate I, Fluorogenic - Calbiochem

A tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec⁻¹, Km = 11.2 µM, and kcat/Km = 1.9 x 10⁶ M⁻¹ sec⁻¹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ac-HRYR-ACC

219497
  
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      Overview

      Replacement Information
      Description
      OverviewA tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec-1, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M-1 sec-1). Displays ~270-fold and ~2,500-fold greater selectivities over cathepsin B (kcat/Km = 7.0 x 103 M-1 sec-1),respectively and cathepsin K (kcat/Km = 7.6 x 102 M-1 sec-1). An excitation wavelength of 380 nm and an emission wavelength of 460 nm is recommended in order to maximize the signal of the ACC group over the background signal of uncleaved substrate.

      This product has been discontinued.
      ACC = 7-Amino-4-carbamoylmethylcoumarin.
      Catalogue Number219497
      Brand Family Calbiochem®
      SynonymsAc-HRYR-ACC
      References
      ReferencesChoe, Y., et al. 2006. J. Biol. Chem. 281, 12824.
      Product Information
      ATP CompetitiveN
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₄₀H₅₂N₁₄O₉
      Chemical formulaC₄₀H₅₂N₁₄O₉
      Hygroscopic Hygroscopic
      ReversibleN
      Applications
      Biological Information
      Primary TargetHuman cathepsin L
      Primary Target IC<sub>50</sub>kcat = 20.8 sec⁻¹, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M⁻¹ sec⁻¹ as substrate for human cathepsin LL
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceAc-His-Arg-Tyr-Arg-ACC
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      219497 0

      Documentation

      Cathepsin L Substrate I, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      219497

      References

      Reference overview
      Choe, Y., et al. 2006. J. Biol. Chem. 281, 12824.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-September-2007 RFH
      SynonymsAc-HRYR-ACC
      DescriptionA tetrapeptidyl fluorogenic substrate for human cathepsin L (kcat = 20.8 sec-1, Km = 11.2 µM, and kcat/Km = 1.9 x 106 M-1 sec-1). Displays ~270-fold and ~2,500-fold greater selectivities, respectively, over cathepsin B (kcat/Km = 7.0 x 103 M-1 sec-1) and cathepsin K (kcat/Km = 7.6 x 102 M-1 sec-1). An excitation wavelength of 380 nm and an emission wavelength of 460 nm is recommended in order to maximize the signal of the ACC group over the background signal of uncleaved substrate.
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₄₀H₅₂N₁₄O₉
      Peptide SequenceAc-His-Arg-Tyr-Arg-ACC
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml) or 5% Acetic acid (5 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesChoe, Y., et al. 2006. J. Biol. Chem. 281, 12824.