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207007 Calcineurin Cellular Activity Assay Kit, Colorimetric

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PP2B Assay Kit, Colorimetric

207007
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Overview

Key Spec Table

Species ReactivityDetection Methods
H, M, RColorimetric

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207007-1KTT
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      Description
      OverviewA colorimetric assay kit for measuring cellular calcineurin (PP2B) enzyme activity. Assays are performed in a convenient 96-well plate format with all reagents necessary for measuring calcineurin activity. The RII Phosphopeptide Substrate (Cat. No. 207008), supplied with this kit, is the most efficient and selective substrate known for calcineurin. The free phosphate released is detected using malachite green.
      Catalogue Number207007
      Brand Family Calbiochem®
      SynonymsPP2B Assay Kit, Colorimetric
      Application Data
      Materials Required but Not Delivered• Plate reader capable of measuring A620 to ≥3-decimal accuracy.
      • Centrifuge capable of 100,000 x g RCF.
      • Swing bucket centrifuge capable of 800 x g RCF.
      • Pipettor capable of pipetting 5-100 µl accurately.
      • Multi-channel pipettor capable of pipetting 100 µl (optional).
      • Ice bucket to keep reagents cold until use.
      • 16 gauge needle/syringe.
      • TBS buffer, 100 ml (150 mM NaCl, 20 mM Tris, pH 7.2)
      • 15 ml conical centrifuge tubes
      • Biological test materials (e.g., tissue, cells)
      References
      ReferencesMondragon, A., et al. 1997. Biochemistry 36, 4934.
      Donella-Deana, A., et al. 1994. Eur. J. Biochem. 219, 109.
      Enz, A., et al. 1994. Anal. Biochem. 216, 147.
      Harder, K.W., et al. 1994. Biochem. J. 298, 395.
      Martin, B., et al. 1985. J. Biol. Chem. 260, 14932.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsHuman Recombinant Calcineurin as a Positive Control, Bovine Brain Calmodulin, Calcineurin Substrate (Cat. No. 207008), Assay Buffer, EGTA Buffer, Lysis Buffer, Protease Inhibitor Cocktail, Phosphate Detection Agent, Phosphate Standard, Okadaic Acid (Cat. No. 495604), Desalting Column Resin, Chromatography Column, ½ volume 96-Well Plate, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time7 h
      Sample TypeTissue/cellular extracts
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Safety Information
      R PhraseR: 21/22-36/38

      Harmful in contact with skin and if swallowed.
      Irritating to eyes and skin.
      S PhraseS: 26-24/25

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Avoid contact with skin and eyes.
      Product Usage Statements
      Intended useThe Calbiochem® Calcineurin Cellular Activity Assay Kit is a complete colorimetric assay kit for measuring cellular calcineurin (PP-2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin phosphatase activity in tissue/cellular extracts. Human recombinant calcineurin is included as a positive control. The RII phosphopeptide substrate, supplied with this kit is the most efficient and outstanding peptide substrate known for calcineurin. The detection of free phosphate released is based on the malachite green assay and offers the following advantages: non-radioactive, convenient one-step detection, and excellent sensitivity.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsStore all components except the plate (room temperature) at -70°C for the highest stability. The calcineurin enzyme component must be handled particularly carefully in order to retain maximal enzymatic activity. Thaw it quickly in a room temperature water bath or by rubbing between your fingers, then immediately store on ice. The remaining unused enzyme should be quickly refrozen by placing at -70°C. To minimize the number of freeze/thaw cycles, aliquot the calcineurin into separate tubes and store at -70°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsHuman Recombinant Calcineurin as a Positive Control, Bovine Brain Calmodulin, Calcineurin Substrate (Cat. No. 207008), Assay Buffer, EGTA Buffer, Lysis Buffer, Protease Inhibitor Cocktail, Phosphate Detection Agent, Phosphate Standard, Okadaic Acid (Cat. No. 495604), Desalting Column Resin, Chromatography Column, ½ volume 96-Well Plate, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      207007-1KTT 04055977203226