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CBA053 COX-2 ELISA Kit

COX-2 ELISA Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms:  Cyclooxygenase-2 ELISA Kit

Detection Methods: Colorimetric
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CBA053
  
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      Overview

      Key Spec Table

      Species ReactivityDetection Methods
      HColorimetric
      Description
      Overview

      This product has been discontinued.



      This kit detects and quantifies human COX-2 in cell lysates and tissue extracts. Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins from arachidonic acid. COX-2 expression induced by various inflammatory and/or mitogenic stimuli such as cytokines, growth factors or tumor promoters.
      Catalogue NumberCBA053
      Brand Family Calbiochem®
      Synonyms Cyclooxygenase-2 ELISA Kit
      Application Data
      Example standard curve using the COX-2 Standard. A standard curve was generated according to the COX-2 Standard preparation and the Detailed Protocol above. The results represent a typical standard curve. Assay range: 2.5-200 ng/ml. Lower limit of detection: ~2 ng/ml.
      Materials Required but Not Delivered• Precision pipettors with disposable tips
      • Wash bottle or multi-channel dispenser for washing
      • COX-2 inducer (see Preparation of Samples below)
      • CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) or equivalent cell lysis buffer, preferably with protease inhibitors (e.g. Protease Inhibitor Cocktail Set III, Cat. No. 539134)
      • Protein assay (e.g. Non-Interfering Protein Assay™, Cat. No. 488250 or BCA Protein Assay Kit, Cat. No. 71285)
      • Spectrophotometer capable of measuring absorbance in 96-well plates at 450 nm, preferably with a reference wavelength of 540-600 nm
      References
      ReferencesBlanke, S.R., et al. 2005. Semin. Oncol. 32, 69.
      Dannenberg, A.J., et al. 2005. J. Clin. Oncol. 23, 254.
      Sanborn, R. and Blake, C.D., 2005. Semin. Oncol. 32, 69.
      Kim, H.J., et al. 2003. Int. J. Oncol. 22, 99.
      Shigemasa, K., et al. 2003. Int. J. Oncol. 22, 99.
      El-Bayoumy, K., et al. 2002. Int. J. Oncol. 20, 557.
      Hwang, D., et al. 1998. J. Natl. Cancer Inst. 90, 455.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsAnti-COX-2 Coated 96-Well Plate, COX-2 Standard, COX-2 Detector Antibody, HRP Conjugate, Assay Diluent, ELISA 20X Plate Wash Concentrate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol
      Positive controlU937 cells
      Biological Information
      Assay range2.5-200 ng/ml
      Sample TypeCell lysate, tissue extract
      Species Reactivity
      • Human
      Product Usage Statements
      Intended useThe Calbiochem® COX-2 ELISA Kit is intended for the quantitative measurement of COX-2 in human cell lysates. Serum and plasma samples are not recommended due to inherently low concentrations of COX-2 in biological fluids.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsAnti-COX-2 Coated 96-Well Plate, COX-2 Standard, COX-2 Detector Antibody, HRP Conjugate, Assay Diluent, ELISA 20X Plate Wash Concentrate, TMB Substrate, ELISA Stop Solution, Plate Sealers, and a user protocol
      Global Trade Item Number
      Catalogue Number GTIN
      CBA053 0