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14-773M BTK (E41K) Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.

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14-773M
250 µg  Also available in 10 μg size (cat#14-773) and in bulk (cat# 14-773-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-773M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionBTK (E41K) Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant, full-length, human BTK
      Background InformationSpecific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • BTK
      • ATK
      • BPK
      • AGMX1
      Protein TargetBTK
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      Molecular Weight78.4kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of LKBtide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-773) and in bulk (cat# 14-773-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-773M 04053252324017

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-773 BTK (E41K) Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
      Learn More >>

      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases