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391334 BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

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391334
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
10191-18-1C₆H₁₅NO₅S

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391334-100GM
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      391334-25GM
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          Description
          OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
          Absorbance (100 mM, 260 nm):≤0.05.
          Catalogue Number391334
          Brand Family Calbiochem®
          SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
          References
          ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
          Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
          Product Information
          CAS number10191-18-1
          FormWhite solid
          Hill FormulaC₆H₁₅NO₅S
          Chemical formulaC₆H₁₅NO₅S
          PKA7.12 at 20°C
          Quality LevelMQ100
          Applications
          Biological Information
          Purity≥99% by titration (dry basis)
          Physicochemical Information
          Heavy metals≤1 ppm
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSKI7363500
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          391334-100GM 07790788058364
          391334-25GM 04055977190083

          Documentation

          BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem Certificates of Analysis

          TitleLot Number
          391334

          References

          Reference overview
          De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
          Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-July-2008 RFH
          SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
          DescriptionBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
          FormWhite solid
          CAS number10191-18-1
          RTECSKI7363500
          Chemical formulaC₆H₁₅NO₅S
          Purity≥99% by titration (dry basis)
          SolubilityH₂O (1 mg/ml)
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
          Toxicity Standard Handling
          ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
          Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.