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14-835M Aurora B Protein, active, 250 µg

Active, His-tagged, recombinant, human Aurora B, full length & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end, for use in Kinase Assays.

Aurora B Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-835M
250 µg  Also available in 1 μg size (cat#14-835) and in bulk (cat# 14-835-K).
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      Overview

      Description
      Catalogue Number14-835M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAurora B Protein, active, 250 µg
      OverviewHis-tagged, recombinant, human Aurora B, full length and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, His-tagged, recombinant, human Aurora B, full length & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourcehuman Aurora B, human INCENP co-expressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryChromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM]
      Gene Symbol
      • AIM-1
      • STK12
      • Aik2
      • IPL1
      • Aurora-B
      • ARK2
      • aurora-B
      • AIK2
      • AIM1
      • AurB
      • STK5
      • aurora-1
      • STK-1
      • AURKB
      Protein TargetAurora-B
      Purification MethodGlutathione-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96GD4 # May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis.
      COFACTOR: Magnesium.
      SIZE: 344 amino acids; 39280 Da
      SUBUNIT: Interacts with TACC1. Associates with RACGAP1 during M phase. Component of the CPC at least composed of BIRC5/survivin CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with CDCA1 and KNTC2. Interacts with EVI5.
      SUBCELLULAR LOCATION: Nucleus. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid- body.TISSUE SPECIFICITY: High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.DISEASE:SwissProt: Q96GD4 # Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
      SIMILARITY: SwissProt: Q96GD4 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. & Contains 1 protein kinase domain.
      Molecular WeightAurora B = 40 kDa
      INCENP = 38 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 1 μg size (cat#14-835) and in bulk (cat# 14-835-K).
      Global Trade Item Number
      Catalogue Number GTIN
      14-835M 04053252675126