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OP07A Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP07A
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
      Catalogue NumberOP07A
      Brand Family Calbiochem®
      References
      ReferencesCourtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Product Information
      FormLiquid suspension
      FormulationIn PBS.
      Negative controlHT1080 or NIH/3T3 cells
      Positive controlCOLO-201 cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (Use Cat. No. OP07 or OP07L, see application references)
      Immunoprecipitation (15 µl beads per sample, see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      Application Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src that can be used for in vitro kinase assays (see application references. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified v-Src
      ImmunogenHuman
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP07A 0

      Documentation

      Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate Certificates of Analysis

      TitleLot Number
      OP07A

      References

      Reference overview
      Courtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationImmunoblotting (Use Cat. No. OP07 or OP07L, see application references)
      Immunoprecipitation (15 µl beads per sample, see application references)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Conjugated to agarose. Recognizes the ~60 kDa Src protein.
      BackgroundThe major translational product of the avian sarcoma virus has been designated pp60src. Avian sarcoma virus pp60src possesses a characteristic protein kinase activity with specificity for tyrosine residues. The mammalian cellular homologue of pp60src, designated pp60c-src, exhibits a similar protein tyrosine kinase activity which can be activated by interaction with polyoma virus-encoded middle T antigen. Immunoprecipitates of middle T antigen also contain phosphatidylinositol kinase activity coinciding with the presence of a 81 kDa (p81) protein. The p81 is phosphorylated by activated pp60c-src and is thought to be the phosphatidylinositol kinase.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified v-Src
      Epitopewithin the SH3 domain of v-Src and c-Src
      Clone327
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlCOLO-201 cells
      Negative controlHT1080 or NIH/3T3 cells
      FormLiquid suspension
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src that can be used for in vitro kinase assays (see application references. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCourtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
      Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
      Courtneidge, S.A. 1985. EMBO J. 4, 1471.
      Whitman, M., et al. 1985. Nature 315, 239
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
      Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
      Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
      Collett, M.S., et al. 1978. Cell 15, 1363.
      Application referencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.

      Related Products & Applications

      Alternative Products

      Catalogue Number Description  
      OP07 Anti-v-Src (Ab-1) Mouse mAb (327) Show Pricing & Availability
      OP07L Anti-v-Src (Ab-1) Mouse mAb (327) Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies