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ABN2268 Anti-proSAAS

Anti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-proSAAS MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Pcsk1n UniProt Number: Q9QXV0
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ABN2268
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICC, IF, WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABN2268
      DescriptionAnti-proSAAS
      Alternate Names
      • IA-4
      • Proprotein convertase subtilisin/kexin type 1 inhibitor
      • Proprotein convertase 1 inhibitor
      • pro-SAAS
      Background InformationProSAAS (UniProt: Q9QXV0; also known as IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS) is encoded by the Pcsk1n gene (Gene ID: 30052) in murine species. ProSAAS is a small secretory protein that is primarily expressed in neural and neuroendocrine tissues and acts as an anti-aggregant chaperone. Its presence appears to be restricted only to vertebrates. Mammalian proSAAS does not undergo either glycosylation or phosphorylation, but undergoes proteolytic cleavage at several pairs of basic residues that leaves its core region intact. ProSAAS is known to interact with proprotein convertase subtilisin/kexin type 1 (PCSK1) via the C-terminal inhibitory domain (aa 219-258). In the neural tissues proSAAS exhibits neuroprotective effects and is shown to block alpha-synuclein-induced cytotoxicity in cultures of dopaminergic neurons and in SH-SY5Y cells. In vitro studies have shown that proSAAS can also prevent the fibrillation of amyloid beta 1-42 peptide in a dose-dependent manner and residues 97-180 are sufficient for its anti-aggregation function against amyloid peptides. Inclusion of recombinant proSAAS in the medium of Neuro2a cells is shown to block the neurocytotoxic effect of amyloid peptide 1-42.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected proSAAS in AtT-20 cells. (Courtesy of Dr. Iris Lindberg @ University of Maryland).

      Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).

      Western Blotting Analysis: A representative lot detected proSAAS in A immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Shakya, M., et. al. (2020 Nov) Cell Stress Chaperones. 25(6): 929–941). Notes: Before probing with Anti-proSAAS, cross link all blots with 1% glutaraldehyde in phosphate-buffered saline(PBS) for 15min, wash well with PBS to remove glutaraldehyde, followed by blocking step. This cross-linking significantly increases the proSAAS signal and reduces the background.

      Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to first 180 amino acids from murine ProSAAS.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects proSAAS in human, mouse, and rat cells. It targets an epitope within the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, and Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pcsk1n
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~19 kDa observed; 27.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in His-tagged mouse proSAAS recombinant protein.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein. Note: For most biological samples a range of 1:1000 for Western blotting and 1:500 for IHC is recommended.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN2268 04054839362897