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06-1303 Anti-phospho-ZAP-70 (Tyr493) Antibody

Anti-phospho-ZAP-70 (Tyr493) Antibody is an antibody against phospho-ZAP-70 (Tyr493) for use in WB.

Anti-phospho-ZAP-70 (Tyr493) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: EC 2.7.10.2, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70 UniProt Number: P43403
06-1303
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1303
      DescriptionAnti-phospho-ZAP-70 (Tyr493) Antibody
      Alternate Names
      • 70 kDa zeta-associated protein
      • Syk-related tyrosine kinase
      • zeta-chain (TCR) associated protein kinase (70 kD)
      • zeta-chain (TCR) associated protein kinase 70kDa
      • zeta-chain associated protein kinase 70kDa
      • zeta-chain associated protein kinase, 70kD
      Background InformationZeta-chain-associated protein kinase 70 (ZAP-70) is a member of the protein tyrosine kinase family. ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Untreated and H2O2-treated Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-ZAP-70 (Tyr493) Antibody is an antibody against phospho-ZAP-70 (Tyr493) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to residues surrounding phosphorylated Tyr493 of ZAP-70.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes ZAP-70 at and around phosphorylated Tyr493.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
      Gene Symbol
      • EC 2.7.10.2
      • FLJ17670
      • FLJ17679
      • SRK
      • STD
      • TZK
      • ZAP-70
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT STRUCTURE: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB By similarity. Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 By similarity. Interacts with FCRL3.
      SUBCELLULAR LOCATION: Cytoplasm By similarity. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic By similarity.
      TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
      DOMAIN: The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
      PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
      INVOLVEMENT IN DISEASE: Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
      Molecular Weight~70 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and H2O2-treated Jurkat cell lysate.
      Western Blot Analysis: A 0.5-1 µg/mL dilution of this antibody detected phosphorylated ZAP-70 in 10 µg of untreated and H2O2-treated Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      06-1303 04053252282843

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies